S100 Calcium Binding Protein A9 Human, Recombinant, His Tag

Synonyms

Calgranulin B, 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14, NIF, P14, Protein S100-A9, S100 calcium-binding protein A9.

S100A9 is part of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100A9 protein is localized in the cytoplasm and/or nucleus of a wide range of cells, and participates in the regulation of several cellular processes such as cell cycle progression and differentiation. S100 genes include no less than 13 proteins which are localized as a cluster on chromosome 1q21. S100A9 is involved in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis.

Description

The Recombinant Human S100A9 produced in E. coli has a molecular mass of 14.35kDa containing 123 amino acid residues of the human S100A9 and fused to a 10 a.a. His tag at N-terminus.

Source

Escherichia coli

Formulation

S100A9 was filtered (0.4 µm) and lyophilized in 0.5 mg/ml in 20mM Tris and 50mM NaCl, pH 7.5.

Solubility

It is recomended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino Acid Sequence

MKHHHHHHAS TCKMSQLERN IETIINTFHQ YSVKLGHPDT LNQGEFKELV RKDLQNFLKK ENKNEKVIEH IMEDLDTNADTCKMSQLERN IETIINTFHQ YSVKLGHPDT LNQGEFKELV RKDLQNFLKK ENKNEKVIEH IMEDLDTNAD KQLSFEEFIM LMARLTWASH EKMHEGDEGP GHHHKPGLGE GTP

Applications

Western blotting.

Usage

Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

Related Products

S100A1 Human, S100A10 Human, S100A11 Human, S100A12 Human, S100A13 Human, S100A14 Human, S100A16 Human, S100A2 Human, S100A3 Human, S100A4 Human, S100A4 Human, His, S100A5 Human, S100A6 Human, S100A6 Mouse, S100A7 Human, S100A7A Human, S100A8 Human, S100A8 Human, His, S100A9 Human, S100A9 Mouse