Soybean P34 Protein, Recombinant, His Tag

The P34 protein is the main allergen for soybean sensitive humans. Soybean protein P34, a thiol protease belonging to the papain family, is a monomeric allergen having an N-terminal amino acid sequence and amino acid composition identical to that of the seed 34kDa protein. It is an insoluble glycoprotein having a pI of 4.5 and a calculated mass of 28.643 Dalton, representing 2–3% of total soybean protein. Upon glycosylation, the mass will be somewhat larger, resulting in a ~32kDa band in non-reduced SDS PAGE gels. It exhibits no enzymatic function due to an absence of the catalytic cysteine. P34 is stored in storage vacuoles of soybean cotyledons.

Description: The E. coli derived His Tag recombinant protein 36kDa, contains Soybean P34 Protein epitopes 214-261, 351-379 amino acids.

Method: Purified by proprietary chromatographic technique.

Formulation: 50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50% glycerol.

Storage: Soybean P34 His although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

Applications: Use as an antigen in ELISA and Western Blots.

Usage: Bio-world products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chem.

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