Thrombopoietin, Human CHO (TPO)

Recombinant Human Thrombopoietin (TPO)

Synonym: Megakaryocyte colony-stimulating factor, Myeloproliferative leukemia virus oncogene ligand, C-mpl ligand, ML, Megakaryocyte growth and development factor, MGDF, TPO, MKCSF, MPLLG, MGC163194, THPO.

Source: Chinese Hamster Ovarian Cells (CHO)

Description: Thrombopoietin Human Recombinant is approximately 80 kDa, consisting of a 332 amino acid residue with a predicted molecular mass of approximately 35 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 80±10 kDa in SDS-PAGE.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized from a concentrated (1mg/ml) solution in water containing 20mM sodium citrate buffer, pH 6.9.

Solubility: It is recommended to reconstitute the lyophilized Thrombopoietin in sterile water not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized Recombinant TPO although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Recombinant TPO can be stored at 4°C for 2-7 days and -18°C for longer. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Purity: Greater than 90% as obsereved by a0. Analysis by RP-HPLC b) Analysis by SDS-PAGE.

Amino acid sequence: SPAPPACDLR VLSKLLRDSH VLHSRLSQCP EVHPLPTPVL LPAVDFSLGE WKTQMEETKA QDILGAVTLL LEGVMAARGQ LGPTCLSSLL GQLSGQVRLL LGALQSLLGT QLPPQGRTTA HKDPNAIFLS FQHLLRGKVR FLMLVGGSTL CVRRAPPTTA VPSRTSLVLT LNELPNRTSG LLETNFTASA RTTGSGLLKW QQGFRAKIPG LLNQTSRSLD QIPGYLNRIH ELLNGTRGLF PGPSRRTLGA PDISSGTSDT GSLPPNLQPG YSPSPTHPPT GQYTLFPLPP TLPTPVVQLH PLLPDPSAPT PTPTSPLLNT SYTHSQNLSQ EG.

Biological activity: The ED50 as determined by the dose-dependant stimulation of MO7e cells is 1-3 ng/ml, corresponding to a Specific Activity of 1 x 10⁶ IU/mg.