Urate Oxidase (Recombinant)

Recombinant Human Urate Oxidase (UOX)

Source: Escherichia Coli.

Background:

Description: Urate oxidase catalyzes the enzymatic oxidation of uric acid into allantoin, an inactive and soluble metabolite. Recombinant Human Urate Oxidase produced in E.Coli is a tetrameric, non-glycosylated polypeptide chain containing 301 amino acids, having a molecular formula of C1523H2383N417O462S7 and a molecular mass of 34,247 Dalton.

Formulation: Each 1.5mg urate oxidase contains 5mg sucrose, 25mg glycine, 0.1mg Tween-80, 13.6mg Ma2HPO4*12H20 and 0.33mg NaH2PO4*2H20.

Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Solubility: It is recommended to reconstitute the lyophilized Recombinant UOX in sterile 18MO-cm H2O not less than 100 g/ml, which can then be further diluted to other aqueous solutions.

Stability: Lyophilized rUOX although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution Recombinant UOX should be stored at 40C between 2-7 days and for future use below -180C. Please prevent freeze-thaw cycles.

Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing. SDS-PAGE Silver Stained gel.

Biological Activity: The specific activity was found to be 18U/mg. One Unit oxidizes one micromole of uric acid per minute at 30 C, at pH 8.9.

Amino acid sequence: msavkaaryg kdnvrvykvh kdektgvqtv yemtvcvlle geietsytka dnsvivatds ikntiyitak qnpvtppelf gsilgthfie kynhihaahv nivchrwtrm didgkphphs firdseekrn vqvdvvegkg idiksslsgl tvlkstnsqf wgflrdeytt lketwdrils tdvdatwqwk nfsglqevrs hvpkfdatwa tarevtlktf aednsasvqa tmykmaeqil arqqlietve yslpnkhyfe idlswhkglq ntgknaevfa pqsdpnglik ctvgrsslks kl

Endotoxin: Less than 10 ng/1.5mg of Urate Oxidase.

Usage: BioWORLD's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.