2-Iminobiotin Separopore® 6B-CL

Applications:

2-Iminobiotin-Separopore® 6B-CL is used for the purification of avidin/streptavidin conjugates. Proteins coupled to streptavidin are more readily recovered in native form from 2-Iminobiotin-Separopore® than the biotin resin. It is also used for removal of free avidin from streptavidin conjugate preparations. Interaction with avidin and streptavidin is pH dependent: forms stable complex above pH 9.5 and dissociates at pH 4.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

Complexes of streptavidin-fused antigens with biotinylated antibodies targeting receptors on dendritic cell surface: a novel tool for induction of specific T-cell immune responses. Mol Biotechnol. (2012) 51: 221-32.
Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli. Biochem J. (2004) 384: 385-90.
A new europium beta-diketone chelate for ultrasensitive time-resolved fluorescence immunoassays. Anal Biochem. (2002) 311: 57-67.
Production and purification of two recombinant proteins from transgenic corn. Biotechnol Prog. (1998) 14: 149-55.