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Description

AMP Separopore® interacts strongly with NAD+-dependent dehydrogenases and ATP-dependent enzymes. Selective elution with gradients of NAD+ or NADP+ allows the resolution of complex mixtures of dehydrogenase isoenzymes using 5’-AMP Separopore®. The production method involves alkylation of the nucleotide, AMP followed by alkaline rearrangement to yield the corresponding N6-carboxymethyl derivative with subsequent condensation using 1,6-diaminohexane to give N6-[(6-aminohexyl)carbamoylmethyl)].

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Properties

Shelf life

4 years

Storage Temperature

2-8°C

ECCN #

EAR99

Appearance color

White

Appearance form

lyophilized powder

Matrix

Separopore® 4B-CL (highly crosslinked agarose beads, 4%)

Particle Size Range

52 - 165 μm

Matrix Activation

Epoxy

Matrix Attachment

N6

Spacer Arm

1, 6-diaminohexane

Binding Capacity

~ 5-7 mg lactate dehydrogenase / ml drained gel

Suspension/Column/Cartridge

Supplied as lyophilized powder and 1g yields 8-10 ml of gel

pH Stability

4 - 10

Flow Specifications

70 - 140 cm / h

Physical Stability

Negligible volume variation due to changes in pH or ionic strength.

Chemical Stability

Stable to all commonly used aqueous buffers and additives like detergents. Avoid high concentrations of EDTA, urea, guanidine-HCl, chaotropic salts and strong oxidizing agents.