Avidin Separopore® 6B

Applications:

Avidin-Separopore® 6B can be used in a variety of applications for the affinity purification or immobilization of biotinylated macromolecules and complexes (such as proteins associated with biotinylated nucleic acids).

• Binding to biotinylated macromolecules, peptides and complexes
• Hybridization of biotinylated RNA to its complementary DNA and binding to immobilized avidin, with subsequent elution of the single-stranded DNA. Avidin-biotin interaction is very strong.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

Carbonylation of adipose proteins in obesity and insulin resistance: identification of adipocyte fatty acid-binding protein as a cellular target of 4-hydroxynonenal. Mol Cell Proteomics. (2007) 6: 624-37.
Synthesis of novel caspase inhibitors for characterization of the active caspase proteome in vitro and in vivo. J Med Chem. (2006) 49: 7636-45.
Oxaloacetate decarboxylase of Vibrio cholerae: purification, characterization, and expression of the genes in Escherichia coli. Arch Microbiol. (2005) 183: 121-9.
Albumin, a new biomarker of organophosphorus toxicant exposure, identified by mass spectrometry. Toxicol Sci. (2005) 83: 303-12.
Subunit gamma of the oxaloacetate decarboxylase Na(+) pump: interaction with other subunits/domains of the complex and binding site for the Zn(2+) metal ion. Biochemistry. (2002) 41: 1285-92.
Evidence for the cluster model of mitochondrial steroid hydroxylase system derived from dissociation constants of the complex between adrenodoxin reductase and adrenodoxin. Biochem Biophys Res Commun. (2000) 276: 210-5.
The assembly factor Atp11p binds to the beta-subunit of the mitochondrial F(1)-ATPase. J Biol Chem. (2000) 275: 5767-72.
Calcium ion-independent recognition of sialyl and nonsialyl N-acetyllactosamine and Le(x) structures by boar sperm. Arch Biochem Biophys. (2000) 374: 86-92.
In vitro biotinylation provides quantitative recovery of highly purified active lactose permease in a single step. Biochemistry. (1998) 37: 15713-9.
Inhibition of acetyl CoA carboxylase by GTP gamma S. Biochim Biophys Acta. (1998) 1384: 130-40.
Identification of protein phosphatase-1-binding proteins by microcystin-biotin affinity chromatography. J Biol Chem. (1996) 271: 28478-84.
Inactivation of acetyl-CoA carboxylase and activation of AMP-activated protein kinase in muscle during exercise. Am J Physiol. (1996) 270: E299-304.
Properties and localization of a tyrosine phosphorylated form of hexokinase in mouse sperm. Mol Reprod Dev. (1996) 43: 82-93.
Repeating the avidin \"chase\" markedly improved the biodistribution of radiolabelled biotinylated antibodies and promoted the excretion of additional background radioactivity. Eur J Cancer. (1995) 31A: 1689-96.
Synthesis of the oxaloacetate decarboxylase Na+ pump and its individual subunits in Escherichia coli and analysis of their function. Eur J Biochem. (1995) 231: 790-801.
Purification and characterization of rat skeletal muscle acetyl-CoA carboxylase. Eur J Biochem. (1995) 231: 192-8.
Expression of the sodium ion pump methylmalonyl-coenzyme A-decarboxylase from Veillonella parvula and of mutated enzyme specimens in Escherichia coli. J Bacteriol. (1995) 177: 3623-30.
Synthesis and characterization of a biotinylated organophosphorus ester for detection and affinity purification of a brain serine esterase: neuropathy target esterase. Biochem J. (1994) 301: 551-6.
Purification of two active fusion proteins of the Na+-dependent citrate carrier of Klebsiella pneumoniae. FEBS Lett. (1994) 347: 37-41.
Ferredoxin binding site on ferredoxin: NADP+ reductase. Differential chemical modification of free and ferredoxin-bound enzyme. Eur J Biochem. (1993) 216: 57-66.