Butyl Separopore® 4B-CL

A standard aliphatic lyzophobic interaction chromatography media for protein purification. Prepared by chemically stable ether linkage. The ligand contains no charge group and provides true hydrophobic interaction chromatography for down stream processing of proteses.

Application: Butyl-Separopore® 4B-CL is a protein separation media for hydrophobic interaction chromatography. Substances are separated on the basis of their different hydrophobicities. Availabilty as bulk as well as 5mL SuperTrap catridges/

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

N-acetyl-D-neuraminic acid lyase generates the sialic acid for colominic acid biosynthesis in Escherichia coli K1. Biochem J. (1996) 317: 157-65.
High level expression, purification and activation of human dipeptidyl peptidase I from mammalian cells. Protein Expr Purif. (2011) 76: 59-64.
Purification, immobilization, and characterization of a specific lipase from Staphylococcus warneri EX17 by enzyme fractionating via adsorption on different hydrophobic supports. Biotechnol Prog. (2011) 27: 717-23.
Efficient separation of homologous alpha-lactalbumin from transgenic bovine milk using optimized hydrophobic interaction chromatography. J Chromatogr A. (2010) 1217: 3668-73.
Expression and purification of canine granulocyte colony-stimulating factor (cG-CSF). Vet Immunol Immunopathol. (2009) 130: 221-5.
Separation of product associating E. coli host cell proteins OppA and DppA from recombinant apolipoprotein A-I(Milano) in an industrial HIC unit operation. Biotechnol Prog. (2009) 25: 446-53.
Angiotensin I retention behavior on Butyl-Sepharose under linear loading chromatographic conditions. J Chromatogr A. (2009) 1216: 2332-8.
Purification and characterization of a major collagenase from Streptomyces parvulus. Biosci Biotechnol Biochem. (2009) 73: 21-8.
Purification and properties of a chitinase from Penicillium sp. LYG 0704. Protein Expr Purif. (2009) 65: 244-50.
Optimization of hydrophobic interaction chromatography using a mathematical model of elution curves of a protein mixture. J Mol Recognit. (2009) 22: 110-20.
Process development for production of human granulocyte-colony stimulating factor by high cell density cultivation of recombinant Escherichia coli. J Ind Microbiol Biotechnol. (2008) 35: 1643-50.
Advancement in the modeling of pressure-flow for the guidance of development and scale-up of commercial-scale biopharmaceutical chromatography. J Chromatogr A. (2008) 1190: 127-40.
Theory and applications of refractive index-based optical microscopy to measure protein mass transfer in spherical adsorbent particles. J Chromatogr A. (2008) 1188: 242-54.
Isolation of soybean protein P34 from oil bodies using hydrophobic interaction chromatography. BMC Biotechnol. (2008) 8: 27.