CDI-Activated Separopore® 4B

• Pre-activated medium for immobilization of ligands containing primary amines.
• N, N'-Carbonyl diimidazole (CDI)-mediated activation of Separopore® 4B matrix to allow coupling of the primary amino ligands by a stable amide bond formation.
• No charge is introduced into the matrix after the coupling reaction.
• No ligand leakage after coupling.
• Superior stability: Activated matrix is much more resistant to hydrolysis.
• Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids.
• Coupling reaction can also be carried out in organic solvents.
• High coupling efficiency and easy to handle.
• Available in Bulk quantities for down stream processing.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

Affinity purification of plasma proteins: characterization of six affinity matrices and their application for the isolation of human factor VIII. Thromb Haemost. (1989) 61: 234-7.
Homogeneous bivalent insulin receptor: purification using insulin coupled to 1, 1\'-carbonyldiimidazole activated-agarose. Biochem Biophys Res Commun. (1985) 132: 1059-65.