CDI-Activated Separopore® 6B-CL

• Pre-activated medium for immobilization of ligands containing primary amines.
• N, N'-Carbonyl diimidazole (CDI) mediated activation of Separopore® matrix to allow coupling of the primary amino ligands by a stable amide bond formation.
• No charge is introduced into the matrix after the coupling reaction.
• No ligand leakage after coupling.
• Superior stability: Activated matrix is much more resistant to hydrolysis.
• Ideal for immobilization of large protein ligands such as antibodies as well as peptides and nucleic acids.
• Coupling reaction can also be carried out in organic solvents.
• High coupling efficiency and easy to handle.
• Available bulk quantities for down stream processing of proteins.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

Hydrolysis of fibrinogen and plasminogen by immobilized earthworm fibrinolytic enzyme II from Eisenia fetida. Int J Biol Macromol. (2003) 32: 165-71.