Cobalt-Chelated Separopore® 4B-CL

Applications:

Co²⁺-chelated Separopore® 4B-CL is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to metal ions. It is suitable for both laboratory scale purification and large-scale use.

Properties:

• Affinity matrix for native and recombinant proteins.
• Imminodiacetate immobilized matrix charged with Co²⁺ metal ions.
• Ideal for purification of recombinant proteins containing (His)₆-fusion tag.
• It provides effective binding and selectivity for a wide range of proteins.
• High salt concentrations can be used and it withstands effective and rigorous Cleaning-In-Procedure (CIP).

Also available in bulk (Inquire).

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical specifications:

• Autoclavable at 121 °C in 20% ethanol
• Antimicrobial agent: 20% ethanol

References

Expression of truncated tobacco osmotin in Escherichia coli: purification and antifungal activity. Biotechnol Lett. (2011) 33: 539-43.
Polyethylene glycosylation prolongs the circulatory stability of recombinant human butyrylcholinesterase. Chem Biol Interact. (2005) 157-158: 115-21.
Purification and characterization of a soluble bioactive amino-terminal extracellular domain of the human thyrotropin receptor. Biochemistry. (2001) 40: 9860-9.