Copper-Chelated Separopore® 4B-CL

Applications:

Cu²⁺-chelated Separopore® 4B-CL is an imminodiacetate (IDA) immobilized metal affinity chromatography (IMAC) matrix that seperates proteins via their differences in affinity to Cu²⁺ metal ions. It is suitable for both laboratory scale purification and large-scale use.

Properties:

• Affinity matrix for proteins.
• Imminodiacetate immobilized matrix charged with Cu²⁺ metal ions.
• Ideal for purification of recombinant proteins containing (His)₆-fusion tag.
• It provides effective binding and selectivity for a wide range of proteins.
• High salt concentrations can be used and it withstands effective and rigorous Cleaning-In-Procedure (CIP).

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical specifications:

• Autoclavable at 121 °C in 20% ethanol
• Antimicrobial agent: 20% ethanol

References

Affinity of fibroblast growth factors for beta-cyclodextrin tetradecasulfate. Anal Biochem. (1990) 185: 108-11.
Heparin-copper biaffinity chromatography of fibroblast growth factors. J Biol Chem. (1988) 263: 9059-62.
Purification and characterization of a human mammary tumor-derived growth factor. Cancer Res. (1986) 46: 1408-12.