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Description

Applications: Used for the purification of liposomal proteins and glycopeptide antibiotics such as teicoplanin and vancomycin group.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Application

Used for the purification of liposomal proteins and glycopeptide antibiotics such as teicoplanin and vancomycin group.

References

Solid-phase enzyme-receptor assay (SPERA): a competitive-binding assay for glycopeptide antibiotics of the vancomycin class. Clin. Chem. (1985) 31: 1606-1610.
Binding of the glycopeptide antibiotic teicoplanin to D-alanyl-D-alanine-agarose: the effect of micellar aggregates. J. Appl. Biochem. (1985) 7: 133-137.
On-column derivatization of the antibiotics teicoplanin and ristocetin coupled to affinity capillary electrophoresis. Electrophoresis (2003) 24: 808-815.
Affinity partitioning of vancomycin. Biotechnol Bioeng. (1991) 38:535-44

Properties

Storage Temperature

2-8°C

ECCN #

EAR99

Matrix

Separopore® 4B (agarose beads, 4%)

Particle Size Range

52 - 165 μm

Matrix Activation

Cyanogen bromide

Matrix Attachment

Amino

Suspension/Column/Cartridge

Supplied as a suspension in 2M NaCl, 0.02% thimerosal

pH Stability

4 - 9

Flow Specifications

70 - 140 cm / h

Ligand Density

2 – 8 μmol D-alanine / ml gel

Molecular Weight Range

6 x 104 - 2 x 107