EAH Separopore® 4B

Applications

EAH-Separopore® 4B is used to couple molecules containing carboxyl groups to the matrix for affinity chromatography. It was prepared by covalent coupling of 1, 6-diamino hexane to Separopore® 4B via epoxy activation. The free terminal amino group allows for coupling of carboxyl-group containing ligands through amide bond formation by carbodimide-mediated coupling method. Due to the long hydrophilic spacer arm (10-atom), bio-World’s EAH-Separopore® 4B is ideal for immobilization of small molecules which are stable in low pH and contain free carboxyl groups.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical specifications:

• Group to be coupled: -COOH groups
• Chemical linkage of ligand: Stable amide linkage

References

Purification and properties of bovine liver holocarboxylase synthetase. Arch Biochem Biophys. (1994) 313: 8-14.
Sialidase in rabbit blood. Characterization of sialidase purified from rabbit erythrocyte membrane. Eur J Biochem. (1994) 221: 655-64.
Isolation of chromosaponin I-specific antibody by affinity chromatography. Biochem Biophys Res Commun. (2003) 302: 587-92.
Purification of cytochromes P-450(scc) and P-450(17 alpha) by steroid-binding affinity column chromatography. J Biochem Biophys Methods. (2001) 49: 417-31.
Immobilization of the hydantoin cleaving enzymes from Arthrobacter aurescens DSM 3747. J Biotechnol. (2001) 92: 179-86.
Immunoaffinity purification of chlorimuron-ethyl from soil extracts prior to quantitation by enzyme-linked immunosorbent assay. J Agric Food Chem. (2001) 49: 1151-7.
New support for the affinity chromatography of hemoglobin. J Chromatogr B Biomed Sci Appl. (1997) 694: 39-48.
Affinity-purified Escherichia coli J5 lipopolysaccharide-specific IgG protects neutropenic rats against gram-negative bacterial sepsis. J Infect Dis. (1994) 170: 622-9.
Refolding of the isolated extracellular domain of the erythropoietin receptor. Biochem Biophys Res Commun. (1994) 200: 482-8.
Application of poly-L-lysine to purification of leukocyte cathepsin G by affinity chromatography. Chem Pharm Bull (Tokyo). (1992) 40: 1634-6.
Application of N-carboxyalkyl peptides to the inhibition and affinity purification of the porcine matrix metalloproteinases collagenase, gelatinase, and stromelysin. Arch Biochem Biophys. (1991) 287: 240-9.