ECH Separopore® 4B

Highlights:

• Affinity matrix for stable carbodiimide-based coupling of ligands containing amino groups.
• Ligands are coupled in a single-step procedure in the presence of a coupling reagent (not provided) which is a water-soluble carbodiimide, such as N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC).
• The matrix provides a 10-atom spacer arm convenient for coupling of small ligands.
• No blocking reaction is necessary following the coupling reaction.
• Ideal for immobilization of small ligands stable in a low pH and containing primary amines.
• Coupling reaction can be carried out in organic solvent.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical specifications:

• Group to be coupled: -NH₂ groups
• Chemical linkage of ligand: Stable amide linkage

References

Serum amyloid P component in mink, a non-glycosylated protein with affinity for phosphorylethanolamine and phosphorylcholine. Amyloid. (2004) 11: 101-8.
Purification and characterization of a 52-kilodalton immunoglobulin G-binding protein from Streptococcus suis capsular type 2. J Bacteriol. (1995) 177: 3830-6.
Immunogenicity of the N-glycans of peanut peroxidase. Phytochemistry. (1994) 37: 933-40.
Rapid purification of protein phosphatase 2A from mouse brain by microcystin-affinity chromatography. FEBS Lett. (1991) 279: 115-8.