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Description

Highlights:

  • Used in coupling of hydroxyl, amino or thiol groups to Separopore® 6B-CL via a 12-carbon hydrophilic spacer arm.
  • Pre-activated medium for immobilization of ligands containing amino (primary or secondary), hydroxyl or thiol group.
  • Ideal for immobilization of alkali-resistant small ligands such as peptides, amino acids and carbohydrates.
  • Epoxy-activated Separopore® 4B-CL is a pre-activated medium for coupling sugars and other carbohydrates via an ether linkage to a hydroxyl group.
  • Medium has a long hydrophilic 12 atom spacer arm which makes it particularly suitable for immobilization of small molecules.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical specifications:

  • Group to be coupled: -NH2, -OH, or -SH
  • 1g swells to 3 – 4 ml

References

Bioconversion of D-glucose into D-glucosone by glucose 2-oxidase from Coriolus versicolor at moderate pressures. Appl Biochem Biotechnol. (2011) 163: 906-17.
Grass degrading beta-1,3-1,4-D-glucanases from Bacillus subtilis GN156: purification and characterization of glucanase J1 and pJ2 possessing extremely acidic pI. Appl Biochem Biotechnol. (2008) 149: 53-66.
Affinity-purified human immunoglobulin G that binds a lacto-N-neotetraose-dependent lipooligosaccharide structure is bactericidal for serogroup B Neisseria meningitidis. Infect Immun. (2007) 75: 1025-33.
L-Asparaginase from Erwinia Chrysanthemi 3937: cloning, expression and characterization. J Biotechnol. (2007) 127: 657-69.
Immobilized zinc affinity chromatography of pectin hydroxamic acids for purification of trypsin inhibitors from soybean and sweet potato. J Agric Food Chem. (2005) 53: 10219-23.
Monoacetylcurcumin: a new inhibitor of eukaryotic DNA polymerase lambda and a new ligand for inhibitor-affinity chromatography. Biochem Biophys Res Commun. (2005) 337: 1288-95.
Isolation of an N-acetyl-D-glucosamine specific lectin from the rhizomes of Arundo donax with antiproliferative activity. Phytochemistry. (2005) 66: 1933-40.
Novel lectins from rhizomes of two Acorus species with mitogenic activity and inhibitory potential towards murine cancer cell lines. Int Immunopharmacol. (2005) 5: 1470-8.
Detection of specific IgE to quinolones. J Allergy Clin Immunol. (2004) 113: 155-60.
Affinity purification of recombinant protein-tyrosine phosphatase 1B using a highly selective inhibitor. J Chromatogr B Analyt Technol Biomed Life Sci. (2004) 799: 1-8.
Flavonoid glycoside: a new inhibitor of eukaryotic DNA polymerase alpha and a new carrier for inhibitor-affinity chromatography. Biochem Biophys Res Commun. (2003) 301: 480-7.
Glutathione S-transferase isoenzymes in the two-spot ladybird, Adalia bipunctata (Coleoptera: Coccinellidae). Arch Insect Biochem Physiol. (2002) 49: 158-66.
Rapid two-step purification of a recombinant mouse Fab fragment expressed in Escherichia coli. Protein Expr Purif. (2001) 22: 325-9.
Substitutions of Thr-103-Ile and Trp-138-Gly in amidase from Pseudomonas aeruginosa are responsible for altered kinetic properties and enzyme instability. Mol Biotechnol. (2001) 17: 201-12.
Development of a tRNA-dependent in vitro translation system. RNA. (2001) 7: 765-73.
Glutathione S-transferases of Aulacorthum solani and Acyrthosiphon pisum: partial purification and characterization. Comp Biochem Physiol B Biochem Mol Biol. (2001) 129: 165-71.
Purification and characterization of rat testicular glutathione S-transferases: role in the synthesis of eicosanoids. Asian J Androl. (2000) 2: 277-82.
Substitution of Glu-59 by Val in amidase from Pseudomonas aeruginosa results in a catalytically inactive enzyme. Mol Biotechnol. (2000) 16: 5-16.
Synthesis and evaluation of N-acetylneuraminic acid-based affinity matrices for the purification of sialic acid-recognizing proteins. Glycoconj J. (1998) 15: 663-9.

Properties

Shelf life

5 year

Storage Temperature

2-8°C

ECCN #

EAR99

Hazmat Ship

Non-hazardous

Appearance color

White

Appearance form

Powder

Matrix

Separopore® 6B-CL (crosslinked agarose beads, 6%)

Particle Size Range

52 - 165 μm

Matrix Activation

Epoxy

Matrix Attachment

Ether

Spacer Arm

12 atoms (when ligands are coupled to free epoxy groups. Linkage is stable, uncharged ether)

Suspension/Column/Cartridge

Supplied as lyophilized powder stabilized with lactose and dextran

pH Stability

2 - 11 (ligand dependent)

Coupling Conditions

pH 9 - 10 (amino ligands), pH 11 - 12 (hydroxyl ligands), pH 7.5 - 8.5 (thiol ligands); Temp: 4 - 37 °C; Time: 16 h to several days; organic solvents possible

Molecular Weight Range

6 x 104 - 2 x 107