Galactose Separopore® 4B-CL (Divinyl Sulfone-Coupled)

Applications

Galactose coupled Separopore® is a high strength bioagarose matrix. Galactose-Separopore® 4B-CL is used in the purification of agglutinins, toxins, lectins, galactosidases and other galactose-binding molecules, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins. This purification method is milder and/or more specific than other methods, allowing protein recovery in a manner that preserves activity for downstream applications.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

Affinity purification of recombinant cholera toxin B subunit oligomer expressed in Bacillus brevis for potential human use as a mucosal adjuvant. FEMS Immunol Med Microbiol. (1998) 20: 311-8. Characterization and purification of the discoidin domain-containing protein retinoschisin and its interaction with galactose. Biochemistry. (2008) 47: 9098-106. A crude extract of Artocarpus integrifolia contains two lectins with distinct biological activities. Immunol Lett. (1992) 31: 65-71. Two asialoglycoprotein receptor polypeptides in human hepatoma cells. J Biol Chem. (1987) 262: 11825-32. Identification of rat testis galactosyl receptor using antibodies to liver asialoglycoprotein receptor: purification and localization on surfaces of spermatogenic cells and sperm. J Cell Biol. (1989) 108: 367-75. A D-galactose-binding lectin with mitogenic activity from Musca domestica pupae. Zoolog Sci. (2009) 26: 249-53. Autoantibodies to asialoglycoprotein receptor (ASGPR) measured by a novel ELISA--revival of a disease-activity marker in autoimmune hepatitis. Clin Chim Acta. (2009) 408: 19-24. The unique enzymatic function of field bean (Dolichos lablab) D-galactose specific lectin: a polyphenol oxidase. Glycoconj J. (2009) 26: 535-45. The amino acids involved in the distinct carbohydrate specificities between macrophage galactose-type C-type lectins 1 and 2 (CD301a and b) of mice. Biochim Biophys Acta. (2008) 1780: 89-100. Balamuthia mandrillaris interactions with human brain microvascular endothelial cells in vitro. J Med Microbiol. (2007) 56: 1110-5. Differential structure and activity between human and mouse intelectin-1: human intelectin-1 is a disulfide-linked trimer, whereas mouse homologue is a monomer. Glycobiology. (2007) 17: 1045-51. Isolation, characterization, and extra-embryonic secretion of the Xenopus laevis embryonic epidermal lectin, XEEL. Glycobiology. (2005) 15: 281-90. One- and two-dimensional gel electrophoresic identification of African yam bean seed proteins. J Agric Food Chem. (2000) 48: 2296-9. Isolation and partial characterisation of galactose-specific lectins from African yam beans, Sphenostyles stenocarpa Harms. Phytochemistry. (1999) 51: 721-8. Molecular cloning and expression of cDNA encoding human macrophage C-type lectin. Its unique carbohydrate binding specificity for Tn antigen. J Immunol. (1996) 156: 128-35. Surfactant protein A amino acids Glu195 and Arg197 are essential for receptor binding, phospholipid aggregation, regulation of secretion, and the facilitated uptake of phospholipid by type II cells. J Biol Chem. (1994) 269: 29801-7. Purification and characterization of Silurus asotus (catfish) roe lectin. Biol Pharm Bull. (1993) 16: 1-5. Cloning, sequence analysis and expression in Escherichia coli of the cDNA encoding a precursor of peanut agglutinin. FEBS Lett. (1992) 307: 185-9. Activation of T and B cells by a crude extract of Artocarpus integrifolia is mediated by a lectin distinct from jacalin. J Immunol Methods. (1991) 140: 197-203. Carbohydrate-recognition domains as tools for rapid purification of recombinant eukaryotic proteins. Biochem J. (1991) 274: 575-80. Purification and characterization of a new type lactose-binding Ulex europaeus lectin by affinity chromatography. Biol Chem Hoppe Seyler. (1991) 372: 95-102. Purification and characterization of two types of Cytisus sessilifolius anti-H(O) lectins by affinity chromatography. Biol Chem Hoppe Seyler. (1991) 372: 103-11. Comparative studies of carbohydrate-binding proteins from Xenopus laevis skin and eggs. Sugar-binding specificities and affinity purification. Chem Pharm Bull (Tokyo). (1990) 38: 975-81. The rat liver asialoglycoprotein receptor polypeptide must be inserted into a microsome to achieve its active conformation. J Biol Chem. (1986) 261: 4940-7. Cytotonic enterotoxins and cytotoxic factors produced by Salmonella enteritidis and Salmonella typhimurium. Toxicon. (1983) 21: 785-96.