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Description

Red Separopore® Anti-Rabbit IgG (H+L) Antibody (Polyclonal IgG) (Agarose)

Cat# 20181081


Details

Host: Goat

Isotype: IgG

Class: Polyclonal

Immunogen: Rabbit IgG (H+L)

Applications:

Goat Anti-Rabbit IgG (H+L) -Red Separopore® 4B binds rabbit IgG and associated antigen-containing proteins to the red colored beads for (co)-immunoprecipitation experiments. Affinity isolated antibody goat anti-rabbit IgG coupled to cyanogen bromide-activated Red Separopore® offers the advantage of increased sensitivity for rabbit IgG without cross-reactivity to other substance present on the membrane or cell surface. Goat Anti-Rabbit IgG (H+L) -Red Separopore® 4B is used for immunoprecipitation and Co-immunoprecipitation studies.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

Immobilization of Saccharomyces cerevisiae cells to protein G-Sepharose by cell wall engineering. J Mol Microbiol Biotechnol. (2003) 5: 161-6.
Affinity-purified Escherichia coli J5 lipopolysaccharide-specific IgG protects neutropenic rats against gram-negative bacterial sepsis. J Infect Dis. (1994) 170: 622-9.
Detection of glycophorin A-like glycoproteins on the surface of cultured human cells. Int J Biochem. (1989) 21: 635-43.
The isolation of a rat alveolar macrophage lectin. J Biol Chem. (1986) 261: 7440-4.
A micro-scale method for the conjugation of affinity-purified Fab\' to beta-D-galactosidase from Escherichia coli. J Biochem. (1985) 98: 1387-94.
Cross-reactivity of contact site A to antibody produced against a stage-specific antigen from a phenol/water extract of Dictyostelium discoideum. J Biochem. (1984) 95: 1355-66.
A lymphocyte cell surface molecule that is antigenically related to actin. J Immunol. (1983) 131: 370-7.

Properties

Shelf life

1 year

Storage Temperature

2-8°C

ECCN #

EAR99

Hazmat Ship

Non-hazardous

Matrix

Red Separopore® 4B (agarose beads, 4%)

Particle Size Range

52 - 165 μm

Matrix Activation

Cyanogen bromide

Binding Capacity

1 mg rabbit IgG / ml drained gel

Suspension/Column/Cartridge

Supplied as 50% slurry in PBS pH 7.4, 0.02% sodium azide

pH Stability

4 - 9 (for use), 2 - 10 (for cleaning)

Flow Specifications

70 - 140 cm / h

Ligand Density

~ 2.4 mg IgG / ml drained gel

Molecular Weight Range

6 x 104 - 2 x 107