Goat Anti-Rabbit IgG (H&L) Separopore® 4B

Separopore® 4B Anti-Rabbit IgG (H+L) Antibody (Polyclonal IgG) (Agarose)

Cat# 20181090

Details

Host: Goat

Isotype: IgG

Class: Polyclonal

Immunogen: Rabbit IgG (H+L)

Applications:

Binds rabbit IgG to the beads. Used for immunoprecipitation and Co-immunoprecipitation studies (to separate the IgG’s antigen-containing protein from solution). Affinity isolated goat anti-rabbit IgG coupled to cyanogen bromide-activated Separopore® 4B offers the advantage of increased sensitivity for rabbit IgG without cross-reactivity to other proteins. Goat anti-rabbit IgG Separopore® binds with the heavy and light chains of rabbit IgG, and with the light chains of rabbit IgM and IgA as demonstrated by ELISA and flow cytometry. The cross reactivity with human immunoglobulins is minimal.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

Immobilization of Saccharomyces cerevisiae cells to protein G-Sepharose by cell wall engineering. J Mol Microbiol Biotechnol. (2003) 5: 161-6.
Affinity-purified Escherichia coli J5 lipopolysaccharide-specific IgG protects neutropenic rats against gram-negative bacterial sepsis. J Infect Dis. (1994) 170: 622-9.
Detection of glycophorin A-like glycoproteins on the surface of cultured human cells. Int J Biochem. (1989) 21: 635-43.
The isolation of a rat alveolar macrophage lectin. J Biol Chem. (1986) 261: 7440-4.
A micro-scale method for the conjugation of affinity-purified Fab\' to beta-D-galactosidase from Escherichia coli. J Biochem. (1985) 98: 1387-94.
Cross-reactivity of contact site A to antibody produced against a stage-specific antigen from a phenol/water extract of Dictyostelium discoideum. J Biochem. (1984) 95: 1355-66.
A lymphocyte cell surface molecule that is antigenically related to actin. J Immunol. (1983) 131: 370-7.