KLH Separopore® 4B

Applications:

Affinity matrix with immobilized KLH used for the purification of antibodies generated against peptide-KLH conjugated antigens byremoving antibodies against the KLH portion of the antigen. Anti-KLH antibodies can be removed by solid phase immuno-affinity column chromatography over the KLH- Separopore^® 4B to obtain clean and specific antibodies against desired peptide.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

Generation of antibodies specific to D-mannitol, a unique haptenic allergen, using reductively aminated D-mannose-bovine serum albumin conjugate as the immunogen. Immunobiology. (2007) 212: 119-28.
Anaphylaxis to excipient mannitol: evidence for an immunoglobulin E-mediated mechanism. Clin Exp Allergy. (2004) 34: 1602-9.
Detection of cells producing murine interferon-alpha using antipeptide antibodies. Immunol Cell Biol. (1994) 72: 234-40.
Construction of antigen-specific suppressor T cell hybridomas from spleen cells of mice primed for the persistent IgE antibody formation. J Immunol. (1988) 141: 3270-7.