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Description

Application: For the purification of membrane dicarboxylate binding proteins and other proteins with affinity to glutamate.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Application

For the purification of membrane dicarboxylate binding proteins and other proteins with affinity to glutamate.

References

Effects of lysine and glutamic acid or [corrected] Mg++ on the conformations of C3 and B, and the activation of the alternative complement pathway. Complement. (1987) 4: 110-9.

Properties

Storage Temperature

2-8°C

ECCN #

EAR99

Hazmat Ship

Non-hazardous

Matrix

Separopore® 4B-CL (crosslinked agarose beads, 4%)

Particle Size Range

52 - 165 μm

Matrix Activation

Cyanogen bromide

Matrix Attachment

Amino

Suspension/Column/Cartridge

Supplied as a suspension in 10 mM Tris, 50 mM KCl, 1 mM EDTA with 0.02% thimerosal

pH Stability

4 - 9

Flow Specifications

70 - 140 cm / h

Ligand Density

5 – 10 μmol / ml

Molecular Weight Range

6 x 104 - 2 x 107