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Description

Applications:

IgG (mouse)-Separopore® 4B is an affinity chromatography gel matrix consisting of mouse IgG bound to Separopore® 4B. This matrix is coupled through a stable, leak resistant linkage over a wide pH range. IgG (mouse)-Separopore® 4B is suitable for antibody purification of anti-IgG antibodies or to remove unwanted cross-reactivity.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

Generation of a high-affinity Fcgamma receptor by Ig-domain swapping between human CD64A and CD16A. Protein Eng Des Sel. (2010) 23: 299-309.
Expression and characterization of human N-acetylglucosaminyltransferases and alpha2,3-sialyltransferase in insect cells for in vitro glycosylation of recombinant erythropoietin. J Microbiol Biotechnol. (2008) 18: 383-91.
Prophylactic potential of liposomized integral membrane protein of Plasmodium yoelii nigeriensis against blood stage infection in BALB/c mice. Vaccine. (2007) 25: 2103-11.
Expression and purification of the BmK Mm2 neurotoxin from the scorpion Buthus martensii Karsch and its biological activity test. Protein Expr Purif. (2004) 38: 45-50.
Characterization of Cln3p, the gene product responsible for juvenile neuronal ceroid lipofuscinosis, as a lysosomal integral membrane glycoprotein. J Neurochem. (2003) 87: 1296-308.
N-acetylglucosamine-6-O-sulfotransferase-1: production in the baculovirus system and its applications to the synthesis of a sulfated oligosaccharide and to the modification of oligosaccharides in fibrinogen. J Biochem. (2003) 133: 287-93.
Minimal catalytic domain of N-acetylglucosaminyltransferase V. Glycobiology. (2000) 10: 595-9.
Partially purified Toxoplasma gondii antigens by immunoaffinity chromatography. Korean J Parasitol. (1997) 35: 251-8.
Evidence that human class Theta glutathione S-transferase T1-1 can catalyse the activation of dichloromethane, a liver and lung carcinogen in the mouse. Comparison of the tissue distribution of GST T1-1 with that of classes Alpha, Mu and Pi GST in human. Biochem J. (1997) 326: 837-46.
Pilot scale expression and purification of soluble protein A tagged beta 1,6N-acetylglucosaminyltransferase in CHO cells. Biochem Biophys Res Commun. (1997) 237: 653-8.
Mouse beta-galactoside alpha 2,3-sialyltransferases: comparison of in vitro substrate specificities and tissue specific expression. Glycobiology. (1997) 7: 469-79.
Epitope-tagged gonadotropin-releasing hormone receptors heterologously-expressed in mammalian (COS-1) and insect (Sf9) cells. Mol Cell Endocrinol. (1997) 127: 143-54.
Mutations at positions 11 and 60 of insulin-like growth factor 1 reveal differences between its interactions with the type I insulin-like-growth-factor receptor and the insulin receptor. Eur J Biochem. (1995) 233: 299-309.
C1q-binding immunoglobulin G in MRL/l mice consists of immune complexes containing antibodies to DNA. Clin Immunol Immunopathol. (1995) 75: 140-6.
Characterization of a novel mouse recombinant processing alpha-mannosidase. Glycobiology. (1994) 4: 445-50.
Sandwich enzyme immunoassay for rat retinol-binding protein using antibody against recombinant antigen and its application. Biochem Med Metab Biol. (1993) 49: 164-72.
Rapid isolation and biochemical characterization of rat C1 and C1q. Mol Immunol. (1993) 30: 433-40.
Fc-receptor-mediated targeting of antibody-bearing liposomes containing dideoxycytidine triphosphate to human monocyte/macrophages. J Pharm Pharmacol. (1993) 45: 48-53.
Artificial immunoglobulin G-binding protein mimetic to staphylococcal protein A. Its production and application to affinity purification of immunoglobulin G. J Chromatogr. (1992) 597: 277-83.
Role of saccharides in immunoglobulin--Fc receptor interactions. Folia Microbiol (Praha). (1992) 37: 71-8.

Properties

Storage Temperature

2-8°C

ECCN #

EAR99

Hazmat Ship

Non-hazardous

Matrix

Separopore® 4B (agarose beads, 4%)

Particle Size Range

52 - 165 μm

Matrix Activation

Cyanogen bromide

pH Stability

3 - 9

Ligand Density

1 mg Mouse IgG / ml drained gel