NHS-Activated Separopore® 6B-CL (With Spacer Arm)

NHS-Activated Separopore® provides a valuable tool for affinity purification of antibodies, antigens and other biomolecules.

Applications:

• Rapid immobilization of antibodies and other proteins to the matrix.
• Immobilization of ligands through primary amines to purify recombinant proteins.
• Immobilization of Protein A or Protein G for monoclonal antibody purification.
• Pre-activated medium for immobilization of ligands containing a primary amino group.
• N-hydroxyl succinimide (NHS) ester forms a stable amide (peptide) bond with the primary amines of ligands.
• The stability of an amide bond at high pH (up to pH 13) allows for a very stringent wash protocol during affinity purification using NHS-activated ligand coupling.
• Coupling can be carried out in neutral pH which makes it ideal for immobilization of antibodies, antigens, and other proteins sensitive to pH extremes.
• Coupling reaction can also be carried out in organic solvents.
• Provides a 10 atom spacer arm upon ligand coupling.
• Coupling completed after 2 – 4 h at room temperature.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical specifications:

• Group to be coupled: Primary -NH₂
• Chemical linkage of ligand: Amide bond

References

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Hepatitis B virus core interacts with the host cell nucleolar protein, nucleophosmin 1. J Microbiol. (2009) 47: 746-52.
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Recombinant human insulin receptor substrate-1 protein. Tyrosine phosphorylation and in vitro binding of insulin receptor kinase. J Biol Chem. (1995) 270: 4870-4.