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Ni-IMAC Separopore® 4B

20170005 bioPLUS™, Separopore®

Ni-IMAC Separopore® 4B

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  SKU Pack Size Price
20170005-1 5 mL - 1 X 5ml $167.19


$167.19

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Description

Ni-immobilized metal affinity chromatography (IMAC) columns consist of Ni-Chelated Separopore®matrix conveniently prepacked spin columns for gravity flow chromatography. Ni-IMAC Columns separate proteins via their differences in affinity to nickel metal ions. Ideal for purification of recombinant proteins containing (His)6 fusion tag.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical Specifications

  • Chelating group coupling method: epoxy activation
  • Metal ion capacity: > 20µmol Ni2+/ml gel

    • Properties

    Shelf life

    shelf life

    Storage Temperature

    2-8°C

    ECCN #

    EAR99

    Hazmat Ship

    Non-hazardous

    Matrix

    cross-linked Separopore® 6B

    Particle Size Range

    50-170μm

    Matrix Attachment

    Imminodiacetate immobilized matrix charged with Ni metal ions.

    Spacer Arm

    14 atom

    Binding Capacity

    Approx. 40 mg His-tagged protein/ml medium

    Suspension/Column/Cartridge

    Supplied as suspension in 20% ethanol

    pH Stability

    3-12