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Description

Application: Poly-L-Lysine-Separopore® 4B-CL is used for the purification of negatively charged proteins and biomolecules.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Application

Poly-L-Lysine-Separopore® 4B-CL is used for the purification of negatively charged proteins and biomolecules.

References

References:
Purification and initial characterization of rat interleukin 2. Proc Natl Acad Sci U S A. (1982) 79: 3020-3.
Identity of an ABA-activated 46 kDa mitogen-activated protein kinase from Zea mays leaves: partial purification, identification and characterization. Planta. (2009) 230: 239-51.
Multiple forms of phosphatase from human brain: isolation and partial characterization of affi-gel blue nonbinding phosphatase activities. Neurochem Res. (2001) 26: 425-38.
Multiple forms of phosphatase from human brain: isolation and partial characterization of affi-gel blue binding phosphatases. Neurochem Res. (2000) 25: 107-20.
Purification of porcine heart latent protein phosphatase Fc.M. Prep Biochem. (1992) 22: 199-213.
Purification of cytochrome c oxidase by lysine-affinity chromatography. Protein Expr Purif. (1992) 3: 36-40.
Isolation and characterization of the calcium- and phospholipid-dependent protein kinase (protein kinase C) subtypes from bovine heart. Biochemistry. (1991) 30: 4334-43.
Purification and characterization of cytosolic protein-tyrosine kinase from bovine platelets. Eur J Biochem. (1988) 174: 471-7.
Purification and properties of a protamine kinase and a type II casein kinase from bovine kidney mitochondria. Arch Biochem Biophys. (1988) 262: 574-84.
Active-site directed inactivation of rat ovarian 20 alpha-hydroxysteroid dehydrogenase. Biochem J. (1986) 240: 717-23.
Binding of interleukin 2 to gangliosides. FEBS Lett. (1984) 170: 391-5.
Isolation and purification of a new 105 kDa protein kinase from rat liver nuclei. Biochim Biophys Acta. (1982) 703: 171-9.
Receptors for human alpha interferon: are gangliosides involved? J Interferon Res. 4: 305-14.

Properties

Shelf life

2 years

Storage Temperature

2-8°C

ECCN #

EAR99

Hazmat Ship

Non-hazardous

Appearance color

White

Appearance form

Suspension

Matrix

Separopore® 4B-CL (crosslinked agarose beads, 4%)

Particle Size Range

52 - 165 μm

Matrix Activation

Cyanogen bromide

Matrix Attachment

ε-Amino

Spacer Arm

1 atom

Suspension/Column/Cartridge

Supplied as a suspension in 0.5 M NaCl, 0.02% sodium azide

pH Stability

4 - 9

Flow Specifications

70 - 140 cm/h

Molecular Weight Range

6 x 104 - 2 x 107

Extent of Coupling

4 mg / ml