Protein A Blue Separopore® 4B Microbeads viewIP™

Applications:

The blue-colored agarose bead affinity matrix has immobilized Recombinant Protein A. Convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, it greatly reducing the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant by visual monitoring of the blue beads. This results in greater consistency and more accurate data due to less sample loss. View-IP™ Protein A Blue-Separopore® 4B-CL is a reliable tool to purify monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

References:
Elution relationships to model affinity chromatography using a general rate model. J Mol Recognit. (2012) 25: 571-9.
Extension of the selection of protein chromatography and the rate model to affinity chromatography. J Mol Recognit. (2010) 23: 609-17.
Affinity purification of proteins using expanded beds. J Chromatogr. (1992) 597: 129-45.
Two-dimensional electrophoresis of cerebrospinal fluid and ventricular fluid proteins, identification of enriched and unique proteins, and comparison with serum. J Neurochem. (1984) 43: 1277-85.
Selective binding of heat- and antigen-aggregated IgG to Blue-Sepharose. Radioimmunoassay of circulating immune complexes. J Immunol Methods. (1983) 65: 207-15.
Partial purification of organ-specific neoantigens from human colon and breast cancer by affinity chromatography with human tumour-specific gamma-globulin. Br J Cancer. (1980) 41: 86-99.