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Description

Applications:

  • Protein G binds specifically to Fc regions of many mammalian immunoglobulins.
  • Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants and other biological fluids.
  • These reagents are also commonly used to capture immune complexes generated in immunoprecipitation experiments.
  • Affinity matrix with immobilized protein G (from Streptococcus sp.) by CNBr activation coupling method.
  • Protein G-Separopore® 4B-CL is used for purification and/or fractionation of IgG subclasses which bind poorly to protein-A such as bovine, goat, rat and sheep IgG.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Properties

Shelf life

1 year

Storage Temperature

2-8°C

ECCN #

EAR99

Hazmat Ship

Non-hazardous

Matrix

Separopore® 6B-CL (crosslinked agarose beads, 4%)

Particle Size Range

52 - 165 μm

Matrix Activation

Cyanogen bromide

Binding Capacity

~ 20 mg human IgG / ml drained gel

Suspension/Column/Cartridge

Supplied as suspension in 0.5M NaCl/0.02% sodium azide

pH Stability

3 - 9 (for use), 2 - 10 (for cleaning)

Ligand Density

~ 2 mg Protein-G / ml drained medium

Molecular Weight Range

6 x 104 - 2 x 107