Close

Description

View-IPTM Anti-Mouse IgG (FC) Antibody Red Separopore® 4B


Details

Host: Rabbit 

Isotype: IgG

Class: Polyclonal

Immunogen: Mouse IgG (FC)

Applications:

Rabbit Anti-Mouse IgG (Fc)-Red Separopore® 4B is used for immunoprecipitation and Co-immunoprecipitation studies. The red color makes it easy to track pellets containing bound material. Affinity isolated antibody is coupled to cyanogen bromide-activated Red Separopore® 4B. The antibody preparation is specific for the Fc fragment of mouse IgG when tested against purified mouse IgA, IgG, IgM, Fc and kappa and lamda light chains. Affinity purified rabbit anti-mouse IgG coupled to Separopore® offers the advantage of increased sensitivity for mouse IgG without cross-reactivity to other substance present on the membrane or cell surface.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

References:

Epitope-tagged gonadotropin-releasing hormone receptors heterologously-expressed in mammalian (COS-1) and insect (Sf9) cells. Mol Cell Endocrinol. (1997) 127: 143-54.
Fc-receptor-mediated targeting of antibody-bearing liposomes containing dideoxycytidine triphosphate to human monocyte/macrophages. J Pharm Pharmacol. (1993) 45: 48-53.
Growth of rat-mouse hybridoma cells in immunosuppressed hamsters. An easy and effective method to prepare monoclonal antibodies from heterohybridoma cell lines. J Immunol Methods. (1990) 130: 25-31.
Evidence for interaction of human anti-idiotypic antibodies with CA 125 determination in a patient after radioimmunodetection. Clin Chem. (1990) 36: 164-7.
Monoclonal antibodies for affinity purification of IL-6/IFN- beta 2 and for neutralization of HGF activity.Hybridoma. (1989) 8: 561-7.
Protein A vectorized toxins--II. Preparation and \"in vitro\" cytotoxic effect of protein A-ricin A chain conjugate on antibody coated human tumour cells. Mol Immunol. (1988) 25: 473-7.
The human B cell-associated antigen CD24 is a single chain sialoglycoprotein. J Immunol. (1986) 136: 3779-84.
Purification of functional, determinant-specific, idiotype-bearing murine T cells. J Exp Med. (1978) 148: 915-24.

Properties

Shelf life

1 year

Storage Temperature

2-8°C

ECCN #

EAR99

Matrix

Red Separopore® 4B (agarose beads, 4%)

Particle Size Range

52 - 165 μm

Matrix Activation

Cyanogen bromide

Binding Capacity

0.5 mg Mouse IgG / ml drained gel

Suspension/Column/Cartridge

Supplied as 50% suspenssion in PBS pH 7.4, 0.02% sodium azide

pH Stability

4 - 9 (for use), 2 - 10 (for cleaning)

Flow Specifications

70 - 140 cm / h

Ligand Density

> 5 mg IgG / ml drained gel

Molecular Weight Range

6 x 104 - 2 x 107