Reactive Red Separopore® 4B-CL

Applications:

The immobilized red dye will bind to a wide variety of NADP⁺- and NAD⁺-dependent enzymes, including many kinases, dehydrogenases and transferases. Carboxypeptidase G, interferon, plasminogen and plasminogen activator are examples of other proteins that can be purified on Red-Separopore® 4B-CL. The cross-linked matrix provides a stable, rigid gel even in the presence of dissociating agents.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

References:
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Susceptibility of human pathogenic bacteria to antimicrobial peptides from sesame kernels. Curr Microbiol. (2007) 55: 162-6.
Identification of an alpha-amylase inhibitor from Pterodon pubescens with ability to inhibit cowpea weevil digestive enzymes. J Agric Food Chem. (2007) 55: 4382-7.
An antifungal peptide from passion fruit (Passiflora edulis) seeds with similarities to 2S albumin proteins. Biochim Biophys Acta. (2006) 1764: 1141-6.
Biochemical and genetic characterization of a D(-)-3-hydroxybutyrate dehydrogenase from Acidovorax sp. strain SA1. J Biosci Bioeng. (2004) 97: 78-81.
Toxicity to cotton boll weevil Anthonomus grandis of a trypsin inhibitor from chickpea seeds. Comp Biochem Physiol B Biochem Mol Biol. (2005) 140: 313-9.
A ribonuclease with antimicrobial, antimitogenic and antiproliferative activities from the edible mushroom Pleurotus sajor-caju. Peptides. (2004) 25: 11-7.
Solid-phase enzyme modification via affinity chromatography. J Chromatogr B Analyt Technol Biomed Life Sci. (2003) 794: 311-22.
Contamination of ribosome inactivating proteins with ribonucleases, separated by affinity chromatography on red sepharose. Prep Biochem Biotechnol. (2003) 33: 101-11.
Molecular cloning and characterization of the gene coding for azoreductase from Bacillus sp. OY1-2 isolated from soil. J Biol Chem. (2001) 276: 9059-65.
A carbonyl reductase-catalyzing reduction of N3-phenacyluridine in rabbit liver. Biol Pharm Bull. (2000) 23: 917-21.
Preparation of highly purified momordin II without ribonuclease activity. Life Sci. (1999) 65: 1485-91.
Characterization and purification of an outer membrane metalloproteinase from Pseudomonas aeruginosa with fibrinogenolytic activity. Biochim Biophys Acta. (1999) 1454: 236-50.
The heparin/heparan sulfate-binding site on apo-serum amyloid A. Implications for the therapeutic intervention of amyloidosis. J Biol Chem. (1999) 274: 7172-81.
Enzymological characteristics of the hyperthermostable NAD-dependent glutamate dehydrogenase from the archaeon Pyrobaculum islandicum and effects of denaturants and organic solvents. Appl Environ Microbiol. (1998) 64: 2152-7.
Studies on proteases and alpha-macroglobulin activity in amphibian blood plasma. Indian J Exp Biol. (1997) 35: 1198-202.
Purification and partial characterization of an indomethacin hydrolyzing enzyme from pig liver. Pharm Res. (1996) 13: 1327-35.
Rapid purification of human complement receptor type 1 (CD35, CR1). J Biochem Biophys Methods. (1996) 32: 69-76.
Prostaglandin-E2 9-reductase from corpus luteum of pseudopregnant rabbit is a member of the aldo-keto reductase superfamily featuring 20 alpha-hydroxysteroid dehydrogenase activity. Eur J Biochem. (1995) 234: 264-70.
Rapid purification of native SecA from Escherichia coli: development of a new affinity chromatography procedure. Curr Microbiol. (1994) 29: 323-9.