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Description

Applications:

Separopore®4B is a high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands. It is ideal for both analytical and preparative protein purifications. Separopore®4B is a proven base matrix for coupling affinity ligands. Separopore® 4B provides good separation over a wide molecular weight range. Larger volumes available at discounted prices (enquire).

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

References:
Coupling of glycosaminoglycans to agarose beads (sepharose 4B). Biochem J. (1971) 124: 677-83.
Purification of a calmodulin-binding protein from chicken gizzard that interacts with F-actin. Proc Natl Acad Sci U S A. (1981) 78: 5652-5.
Regulation of CD44-protein 4.1 interaction by Ca2+ and calmodulin. Implications for modulation of CD44-ankyrin interaction. J Biol Chem. (1997) 272: 30322-8.
Thrombin-induced exposure and prostacyclin inhibition of the receptor for factor VIII/von Willebrand factor on human platelets. J Clin Invest. (1982) 69: 1212-22.
Microdomains of distinctive glycoprotein composition in the kidney proximal tubule brush border. J Cell Biol. (1984) 98: 1505-13.
Studies of the prothrombin activation pathway utilizing radioimmunoassays for the F2/F1+2 fragment and thrombin--antithrombin complex. Blood. (1982) 59: 1086-97.
Purification and characterization of a plasminogen activator inhibitor 1 binding protein from human plasma. Identification as a multimeric form of S protein (vitronectin). J Biol Chem. (1988) 263: 15454-61.
Partial purification and properties of a rat brain phospholipase. J Biol Chem. (1979) 254: 9761-5.
Development of a novel immunoradiometric assay exclusively for biologically active whole parathyroid hormone 1-84: implications for improvement of accurate assessment of parathyroid function. J Bone Miner Res. (2001) 16: 605-14.
Purification of the inducible murine macrophage nitric oxide synthase. Identification as a flavoprotein. J Biol Chem. (1991) 266: 22789-91.
Cloning and characterization of novel ficolins from the solitary ascidian, Halocynthia roretzi. J Biol Chem. (2001) 276: 19959-65.
Improvements on the purification of mannan-binding lectin and demonstration of its Ca(2+)-independent association with a C1s-like serine protease. Biochem J. (1996) 319: 329-32.
Human plasma protein C: isolation, characterization, and mechanism of activation by alpha-thrombin. J Clin Invest. (1979) 64: 761-9.
Induction of nerve growth factor receptor in Schwann cells after axotomy. Proc Natl Acad Sci U S A. (1986) 83: 4094-8.
Purification and characterization of a calmodulin-dependent protein kinase that is highly concentrated in brain. J Biol Chem. (1983) 258: 12735-44.
The role of phospholipid and factor VIIIa in the activation of bovine factor X. J Biol Chem. (1981) 256: 3433-42.
Large, detergent-resistant complexes containing murine antigens Thy-1 and Ly-6 and protein tyrosine kinase p56lck. Eur J Immunol. (1993) 23: 825-31.
Human adenosine deaminase. Distribution and properties. J Biol Chem. (1976) 251: 5448-56.
Distinct forebrain and cerebellar isozymes of type II Ca2+/calmodulin-dependent protein kinase associate differently with the postsynaptic density fraction. J Biol Chem. (1985) 260: 9039-46.
The interaction of wheat germ agglutinin with sialoglycoproteins. The role of sialic acid. J Biol Chem. (1979) 254: 4000-8.
A membrane-bound ATPase from Halobacterium halobium: purification and characterization. J Biochem. (1987) 102: 591-8.
Differential subcompartmentation of terminal glycosylation in the Golgi apparatus of intestinal absorptive and goblet cells. J Biol Chem. (1986) 261: 14307-12.
Arachidonate 5-lipoxygenase of guinea pig peritoneal polymorphonuclear leukocytes. Activation by adenosine 5'-triphosphate. J Biol Chem. (1983) 258: 5754-8.
Insulin-sepharose and the dynamics of insulin action. Proc Natl Acad Sci U S A. (1971) 68: 2066-8.
The mechanism of thrombin-induced platelet factor 4 secretion. Blood. (1980) 55: 661-8.

Properties

Shelf life

3 years

Storage Temperature

2-8°C

ECCN #

EAR99

Matrix

Separopore® 4B (agarose beads, 4%)

Particle Size Range

52 - 165 μm

Suspension/Column/Cartridge

Supplied as a slurry in 20% ethanol

pH Stability

4 - 9

Flow Specifications

70 - 140 cm / h

Physical Stability

Negligible volume variation due to changes in pH or ionic strength.

Chemical Stability

Stable to all solutions commonly used in gel filtration including 8M Urea, 6M guanidine hydrochloride

Molecular Weight Range

6 x 104 - 2 x 107