Ubiquitin Separopore® 4B

Applications:

Ubiquitin-Separopore® 4B is useful for binding the ubiquitin activation enzyme (E1), ubiquitin carrier enzymes (E2s), ubiquitin ligases (E3s) and ubiquitin-terminal hydrolyases. Ubiquitin-Separopore® 4B is used in affinity chromatography to study enzymes involved in intracellular protein degradation, calpain and lysosomal proteases, proteomics, and ubiquitination analysis. Ubiquitin- Separopore® 4B has been used in a study to produce evidence for a particulate location of ubiquitin conjugates and ubiquitin-conjugating enzymes in the rabbit brain. Ubiquitin- Separopore® 4B has also been used to study fertilization of the ascidian,Halocynthia roretzi. Ubiquitin is covalently linked to Separopore® beads via primary amine coupling leaving the C-terminus free.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

References:
Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p. RNA. (2006) 12: 292-302.
Conjugation of phenylalanine hydroxylase with polyubiquitin chains catalysed by rat liver enzymes. Biochim Biophys Acta. (2001) 1547: 379-86.
The ubiquityl-calmodulin synthetase system from rabbit reticulocytes: isolation of the ubiquitin-binding first component, a ubiquitin-activating enzyme. Eur J Biochem. (1998) 255: 482-91.
Induction of ubiquitin conjugating enzyme activity for degradation of topoisomerase II alpha during adenovirus E1A-induced apoptosis. Biochem Biophys Res Commun. (1997) 239: 823-9.
Affinity purification and characterization of protein gene product 9.5 (PGP9.5) from retina. Biochem J. (1996) 318: 711-6.
Demonstration of a ubiquitin binding site on murine haemopoietic progenitor cells: implication of ubiquitin in homing and adhesion. Br J Haematol. (1993) 84: 212-8.
Bovine lens epithelial cells have a ubiquitin-dependent proteolysis system. Biochim Biophys Acta. (1993) 1175: 181-7.
A ubiquityl-calmodulin synthetase that effectively recognizes the Ca(2+)-free form of calmodulin. FEBS Lett. (1993) 315: 347-52.
Purification and partial characterization of ubiquitin-activating enzyme from Saccharomyces cerevisiae. FEBS Lett. (1991) 289: 54-8.
The human ubiquitin carrier protein E2(Mr = 17,000) is homologous to the yeast DNA repair gene RAD6. EMBO J. (1990) 9: 1431-5.
Multiple (alpha-NH-ubiquitin)protein endoproteases in cells. J Biol Chem. (1989) 264: 10637-42.
Components of ubiquitin-protein ligase system. Resolution, affinity purification, and role in protein breakdown. J Biol Chem. (1983) 258: 8206-14.
Immunochemical analysis of the turnover of ubiquitin-protein conjugates in intact cells. Relationship to the breakdown of abnormal proteins. J Biol Chem. (1982) 257: 13964-70.
Covalent affinity purification of ubiquitin-activating enzyme. J Biol Chem. (1982) 257: 2537-42.