CAPS Buffer 0.5M, pH 11.0

Synonym: 3-(Cyclohexylamino)-1-propanesulfonic acid, N-Cyclohexyl-3-aminopropanesulfonic acid

- CAPS buffer is a zwitterionic buffer widely used in Western and immunoblotting experiments as well as protein sequencing and identification.
- Used in the electrotransfer of proteins to PVDF or nitrocellulose membranes.
- Has a pH value of 11.0 and pKa of 10.4 at 25°C.
- The high pH of this buffer makes it useful for the transfer of proteins with a pI > 8.5.
- CAPS buffer has very low metal-binding constants and are therefore particularly suited to investigate metal-dependent enzymes.
- CAPS is selected as a highly water soluble buffering reagent with an optimum buffering pH of 10.4 and minimal reactivity with enzymes or proteins, minimal salt effects.

Warning: For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.

RTECS Number: TZ6395000