Citrate Buffer 0.5M, pH 5.0, Sterile

Citrate buffer solution has a 0.5M concentration at a pH of 5.0. Citrate is used as an anticoagulant, a biological buffer, and is often prepared for antigen retrieval of tissue samples. The solution is designed to break protein cross-links, unmasking antigens and epitopes in formalin-fixed and paraffin embedded tissue sections, resulting in enhanced stain intensity of antibodies. Citrate has as a calcium chelator that forms complexes to disrupt the tendency of blood to clot.

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