Griffonia simplicifolia Lectin (GSL I+II) - Pure

Griffonia simplicifolia lectin (GSL I+II) is affinity purified from a woody climbing shrub native to Africa and consists of both GSL I and GSL II. This lectin shows a greater specificity for blood group B than for blood group A1. GSL I+II interacts with pulmonary microvascular endothelial cells but does not readily interact with pulmonary arterial endothelial cells. This binding pattern has been effectively used to enrich cell populations in vitro, exhibiting functional behaviors similar to those observed in vivo.

Recommended Usage: Recommended dilutions of 0.5 – 10 μg / ml in 1XPBS pH 7.4. Rabbit /Human erythrocytes will agglutinate by 1 - 20 μg / ml of GSLI+II.

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Alveolar Endothelium Recognition, Blotting, ELISA, ELLA, Glycobiology.

References

1.Lamb JE, Shibata S, Goldstein IJ. Purification and Characterization of Griffonia simplicifolia Leaf Lectins. Plant Physiol. 1983 Apr;71(4):879-87.
2.Hayes CE, Goldstein IJ. An alpha-D-galactosyl-binding lectin from Bandeiraea simplicifolia seeds. Isolation by affinity chromatography and characterization. J Biol Chem. 1974 Mar 25;249(6):1904-14.
3.Delmotte FM, Goldstein IJ. Improved procedures for purification of the Bandeiraea simplicifolia I isolectins and Bandeiraea simplicifolia II lectin by affinity chromatography. Eur J Biochem. 1980 Nov;112(2):219-23.