Phaseolus vulgaris Lectin (PHA-E+L) - Pure

Affinity Purified Phaseolus vulgaris lectin (PHA E+L, also known as PHA P, P standing for phytohemagglutinin) is affinity purified lectin consists of two isolectins PHA-E and PHA-L, erythroagglutinin and leucoagglutinin, of which both possess strong mitogenic activity. PHA P has a molecular weight of 122 kDa and an isoelectric point between pH 5.2 and pH 6.2. It has a carbohydrate specificity for complex oligosaccharides and elutes with bovine thyroglobulin or acetic acid. This lectin agglutinates blood group A (-SA).
PHA P can be used for mitogenic stimulation to human lymphocytes. It has the potential to induce closer contacts between adjacent cell membranes and has been successfully used for membrane-induced fusion in human oocytes, bovine oocyte, and caprine oocytes. PHA E+L reacts strongly with Galß4GlcNAcß2Manα6(GlcNAcß4)(GlcNAcß4Manα3)Manß4 and Galß4GlcNAcß6(GlcNAcß2Manα3)Manα3 complex sugars. This lectin is used to bind to central nervous system cells such as the HNK-1 antigen.

Phytohemagglutinin was direct evidence for the involvement of bacterial lectins in the initiation of infection, the basis for present attempts in academia and industry to apply carbohydrates for anti-adhesion therapy of such diseases.

Recommended Usage: Recommended dilutions of 0.5 – 10 μg/ml in 20 mM Tris-HCl pH 7.5, 50 mM NaCl, 1 mM CaCl2, 0.5 mM MnCl2. Less than 0.5 ug /ml will agglutinate neuraminidase treated human erythrocytes

Storage Instruction: To prevent degradation and avoid repeated freeze-thaw cycles, aliquot your product into small, single-use volumes and store them at -20°C

Application

Immunohistochemistry, Immunocytochemistry, Bioconjugation, Immunofluorescence studies, Phytohemagglutinin studies, Blotting, Cell typing, Glycobiology.

References

1.RIGAS DA, OSGOOD EE. Purification and properties of the phytohemagglutinin of Phaseolus vulgaris. J Biol Chem. 1955 Feb;212(2):607-15.
2.Wang P, Leng X, Duan J, Zhu Y, Wang J, Yan Z, Min S, Wei D, Wang X. Functional Component Isolated from Phaseolus vulgaris Lectin Exerts In Vitro and In Vivo Anti-Tumor Activity Through Potentiation of Apoptosis and Immunomodulation. Molecules. 2021 Jan 18;26(2):498.
3.Baumgartner P, Raemaekers RJ, Durieux A, Gatehouse A, Davies H, Taylor M. Large-scale production, purification, and characterisation of recombinant Phaseolus vulgaris phytohemagglutinin E-form expressed in the methylotrophic yeast Pichia pastoris. Protein Expr Purif. 2002 Dec;26(3):394-405.
4.Green ED, Baenziger JU. Oligosaccharide specificities of Phaseolus vulgaris leukoagglutinating and erythroagglutinating phytohemagglutinins. Interactions with N-glycanase-released oligosaccharides. J Biol Chem. 1987 Sep 5;262(25):12018-29.
5.Jiang B, Yuan Y, Zhang X, Feng Z, Liu C. Separation and Enrichment of Lectin from Zihua Snap-Bean (Phaseolus vulgaris) Seeds by PEG 600-Ammonium Sulfate Aqueous Two-Phase System. Molecules. 2017 Sep 22;22(10):1596.
6.Jiang B, Wang X, Wang L, Lv X, Li D, Liu C, Feng Z. Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean Phaseolus vulgaris Seeds. Polymers (Basel). 2019 May 2;11(5):785.