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Description

Applications

α-Lactose-Separopore® 4B-CL is used in protein chromatography and affinity chromatography of glycoproteins. α-Lactose-Separopore® 4B-CL has been used to study microneme proteins in apicomplexans and host-parasite cell surface receptors. Lactose-Separopore® 4B-CL may be used to isolate lactose binding proteins, such as lectins and glycoproteins. α-Lactose Separopore® 4B-CL is prepared by coupling via divinyl sulfone.

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Properties

Shelf life

2 years

Storage Temperature

2-8°C

Appearance form

Suspension

Matrix

Separopore® 4B-CL (crosslinked agarose beads, 4%)

Particle Size Range

52 - 165 μm

Matrix Activation

Divinyl Sulfone

Matrix Attachment

Hydroxyl

Spacer Arm

5 atoms

Binding Capacity

20 - 25 mg lectin from Arachis hypogaea / ml drained gel