Gelatin Separopore® 4B

Applications:

Gelatin Separopore^® 4B has been designed for the purification or removal of fibronectin. Gelatin binds specifically to fibronectin, which is a high molecular weight glycoprotein found on the surfaces of many cell types and present in many extracellular fluids including plasma.

Note: Bulk sizes available for preparative applications (Enquire).

Note: Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

Technical specifications:

• Storage: PBS pH 7.4, 0.3M NaCl added with 0.1% sodium azide

References

Monocyte differentiation induced by co-culture with tumor cells involves RGD-dependent cell adhesion to extracellular matrix. Cancer Lett. (2012) 315: 145-52.
Expression of soluble and functional full-length human matrix metalloproteinase-2 in Escherichia coli. J Biotechnol. (2012) 157: 20-4.
Purification and characterization of a gelatinolytic metalloproteinase from the skeletal muscle of red sea bream (Pagrus major). J Agric Food Chem. (2010) 58: 5730-6.
Purification and characterization of recombinant full-length and protease domain of murine MMP-9 expressed in Drosophila S2 cells. Protein Expr Purif. (2010) 72: 87-94.
Molecular heterogeneity of gelatin-binding proteins from human seminal plasma. Asian J Androl. (2010) 12: 363-75.
Co-purified gelatinases alter the stability and biological activities of human plasma fibronectin preparations. J Periodontal Res. (2010) 45: 292-5.
Adiponectin does not bind to gelatin: a new and easy way to purify high-molecular-weight adiponectin from human plasma. J Lipid Res. (2010) 51: 210-5.
Biochemical characterization of the prolyl 3-hydroxylase 1.cartilage-associated protein.cyclophilin B complex. J Biol Chem. (2009) 284: 17641-7.
The rough endoplasmic reticulum-resident FK506-binding protein FKBP65 is a molecular chaperone that interacts with collagens. J Biol Chem. (2008) 283: 31584-90.
Purification of matrix metalloproteinases by column chromatography. Nat Protoc. (2008) 3: 1111-24.
Interaction of pro-matrix metalloproteinase-9/proteoglycan heteromer with gelatin and collagen. J Biol Chem. (2008) 283: 13652-65.
Purification and characterization of gelatinase-like proteinases from the dark muscle of common carp (Cyprinus carpio). J Agric Food Chem. (2008) 56: 2216-22.
A matrix metalloproteinase-9 activation cascade by hepatic stellate cells in trans-differentiation in the three-dimensional extracellular matrix. J Biol Chem. (2007) 282: 12928-39.
High-level expression of a soluble and functional fibronectin type II domain from MMP-2 in the Escherichia coli cytoplasm for solution NMR studies. Protein Expr Purif. (2007) 53: 124-31.
Neutrophil activator of matrix metalloproteinase-2 (NAM). Clin Exp Metastasis. (2006) 23: 259-68.
Cellular response of human cardiac fibroblasts to mechanically simulated aortic regurgitation. Am J Ther. (2006) 13: 8-11.
Isolation and characterization of heparin and gelatin binding buffalo seminal plasma proteins and their effect on cauda epididymal spermatozoa. Anim Reprod Sci. (2005) 90: 243-54.
ADAM 12 cleaves extracellular matrix proteins and correlates with cancer status and stage. J Biol Chem. (2004) 279: 51323-30.
Identification, purification and partial characterization of tissue inhibitor of matrix metalloproteinase-2 in bovine pulmonary artery smooth muscle. Mol Cell Biochem. (2003) 254: 275-87.
BaG, a new dimeric metalloproteinase/disintegrin from the Bothrops alternatus snake venom that interacts with alpha5beta1 integrin. Arch Biochem Biophys. (2003) 416: 171-9.