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Description

Separopore® 4B-CL is crosslinked high gel strength bioagarose matrix for gel filtration and coupling of affinity ligands suitable for analytical and preparative protein purifications. Separopore® 4B-CL is a proven base matrix for coupling affinity ligands. Separopore® 4B-CL provides good separation over a wide molecular weight range. Larger volumes available at discounted prices (enquire). Separopore® is a cost-effective equivalent to Sepharose® in all of its physical properties and binding characteristics.

References

References:
Affinity-column-mediated immunoenzymometric assays: influence of affinity-column ligand and valency of antibody-enzyme conjugates. Clin Chem. (1984) 30: 1494-8.
Multiple gene-like sequences related to the rabbit hepatic progesterone 21-hydroxylase cytochrome P-450 1. J Biol Chem. (1985) 260: 13347-54.
Proteoglycans from adult human gingival epithelium. Biochem J. (1979) 183: 467-70.
Characterization of a major fibroblast cell surface glycoprotein. Biochemistry. (1977) 16: 5552-9.
Photocoagulation of human plasma: acyl serine proteinase photochemistry. Photochem Photobiol. (1990) 51: 37-43.
The immobilization of mitochondrial malate dehydrogenase on Sepharose beads and the demonstration of catalytically active subunits. J Biol Chem. (1981) 256: 2383-8.
Salt-independent binding of antibodies from human serum to thiophilic heterocyclic ligands. J Chromatogr B Biomed Sci Appl. (1998) 709: 189-96.
Identification of a polymorphically expressed member of the human cytochrome P-450III family. Mol Pharmacol. (1989) 36: 97-105.
Two monoclonal antibodies recognizing different epitopes on rat cytochrome IIB1 react with human IIE1. Mol Pharmacol. (1992) 41: 76-82.
Isolation of a five-polypeptide cytochrome b-f complex from spinach chloroplasts. J Biol Chem. (1983) 258: 10348-54.
The 110,000-dalton actin- and calmodulin-binding protein from intestinal brush border is a myosin-like ATPase. J Biol Chem. (1984) 259: 14128-35.
Biotin-binding protein from egg yolk. A protein distinct from egg white avidin. J Biol Chem. (1978) 253: 6979-82.
Degradation of heparin proteoglycan in cultured mouse mastocytoma cells. Biochem J. (1987) 246: 409-15.
Fibronectin in human gallbladder bile: cholesterol pronucleating and/or mucin "link" protein? Am J Physiol. (1994) 267: G393-400.
Monoclonal and polyclonal antibodies recognizing acetaldehyde-protein adducts. Biochem Pharmacol. (1998) 56: 1515-23.

Properties

Shelf life

5 years

Storage Temperature

2-8°C

ECCN #

EAR99

Matrix

Separopore® 4B-CL (crosslinked agarose beads, 4%)

Particle Size Range

52 - 165 μm

Suspension/Column/Cartridge

Supplied as a slurry in 20% ethanol

pH Stability

3 - 12

Flow Specifications

70 - 140 cm / h

Physical Stability

Negligible volume variation due to changes in pH or ionic strength.

Chemical Stability

Stable to all solutions commonly used in gel filtration including 8M Urea, 6M guanidine hydrochloride

Sterilization

Chemical