IPTG

IPTG, or Isopropyl β-d-1-thiogalactopyranoside, induces protein expression controlled by the Lac Operon, most often when induction of β-galactosidase is necessary. Unlike allolactose, its concentration remains constant because it is not consumed by the cell. IPTG is often used alongside X-Gal to visualize recombinant bacteria.

In most molecular biology applications, IPTG is used at a concentration of less than 3mM. bioWORLD's Molecular Biology Grade IPTG is readily soluble in water. Sterilization of the solution via filtration is recommended for best results. Alternatively, a 100mM, 0.5M, or 1M sterile solution may be purchased.

For convenient use in blue-white screening of recombinant bacteria, bioWORLD offers a combination of IPTG and X-Gal that can be sprayed directly on colonies of interest.

bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY. The product may not be used as a drug, agricultural or pesticidal product, food additive or as a household chemical.

References

See "Screening Bacterial Colonies Using X-Gal and IPTG: α-Complementation" and "Development of the Mammalian Expression Vector System that can be Induced by IPTG and/or Lactose" by PubMed.